InterPro domain: IPR011684

This entry represents the NAB domain found in the Networked proteins.

The Networked (NET) proteins are a superfamily of plant-specific actin-bindingproteins which localize simultaneously to the actin cytoskeleton and specificmembrane compartments and are suggested to couple these membranes to the actincytoskeleton in plant cells. The minimal actin binding region, referred to asthe NET actin-binding (NAB) domain, represents a new actin binding motifunique to plants with no apparent primary sequence homolgy to previouslyidentified actin binding domains. In Arabidopsis, the NAB domain always startswith three conserved tryptophan residues, WWW, a motif whose worldwide webconnection gives added significance to the NET family name. The C-terminalhalf of the domain is very highly conserved, more so than the N-terminal[ 1 , 2 ].

The predicted secondary structure of the domain includes three major alphahelices connected by a beta turns with the WWW motif predicted to form a betasheet [ 3 , 3 ].

1. A superfamily of actin-binding proteins at the actin-membrane nexus of higher plants. Curr. Biol. 22, 1595-600
2. The evolution of the actin binding NET superfamily. Front Plant Sci 5, 254