InterPro domain: IPR011665

In budding yeasts, Brf1 forms the TFIIIB complex with TATA-binding protein (TBP) and Bdp1 [ 1 ]. The TFIIIB complex can be recruited to the Pol III promoters and form an exceptionally kinetically stable TFIIIB-DNA complex, which then recruits the Pol III enzymatic complex and helps maintain it for multiple transcription cycles in a process called facilitated recycling [ 2 ].

This entry represents the TATA binding protein (TBP) binding domain of Brf1 that covers both the Brf homology II and III regions [ 3 ].

1. Intergenic transcriptional interference is blocked by RNA polymerase III transcription factor TFIIIB in Saccharomyces cerevisiae. Genetics 196, 427-38
2. Mapping the protein interaction network for TFIIB-related factor Brf1 in the RNA polymerase III preinitiation complex. Mol. Cell. Biol. 34, 551-9
3. Crystal structure of a transcription factor IIIB core interface ternary complex. Nature 422, 534-9