InterPro domain: IPR010506

This is a ~120-amino acid protein-protein interaction module that binds DMAP1 (DNA methyltransferase-associated protein 1), a transcriptional co-repressor. It is found at the N terminus of DNMT1 (DNA methyltransferase 1) [ 1 ] and animal disco-interacting protein 2 (DIP-2), a protein that maintains morphology of mature neurons [ 2 , 3 ]. This domain is also found in N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTA) and gamma (GNPTG) from animals, which are members of a complex that catalyses the initial step in the formation of the mannose 6-phopsphate targeting signal on newly synthesized lysosomal acid hydrolases. The DMAP1-binding domain mediates the selective binding GlcNAc-1-phosphotranferase to acid hydrolases [ 4 , 5 ].

The DMAP1-binding domain is predicted to adopt a long helix-turn-helix structure that is rich in leucine residues [ 6 ].

1. DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci. Nat. Genet. 25, 269-77
2. DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons. Dev Biol 421, 233-244
3. DIP-2 suppresses ectopic neurite sprouting and axonal regeneration in mature neurons. J Cell Biol 218, 125-133
4. The DMAP interaction domain of UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase is a substrate recognition module. Proc Natl Acad Sci U S A 110, 10246-51
5. Analysis of mucolipidosis II/III GNPTAB missense mutations identifies domains of UDP-GlcNAc:lysosomal enzyme GlcNAc-1-phosphotransferase involved in catalytic function and lysosomal enzyme recognition. J. Biol. Chem. 290, 3045-56
6. New 5' regions of the murine and human genes for DNA (cytosine-5)-methyltransferase. J Biol Chem 271, 31092-7