InterPro domain: IPR010033

This entry represents the IIIC subfamily of the Haloacid Dehalogenase (HAD) superfamily of aspartate nucleophile hydrolases. Subfamily III, which includes subfamily IIIA ( IPR006549 ) and subfamily IIIB ( IPR005519 ) contains sequences which do not contain either of the insert domains between the 1st and 2nd conserved catalytic motifs, subfamily I ( IPR006439 , IPR006545 , IPR006434 , and IPR006435 ), or between the 2nd and 3rd, subfamily II ( IPR006357 , and IPR006379 ). Subfamily IIIC contains five relatively distantly related clades: a family of viral proteins ( IPR007827 ), a family of eukaryotic proteins called MDP-1 and a family of archaeal proteins most closely related to MDP-1 ( IPR010036 ), a family of bacteria including the Streptomyces FkbH protein ( IPR010037 ), and a small clade including the Pasteurella multocida BcbF and EcbF proteins. The overall lack of species overlap among these clades may indicate a conserved function, but the degree of divergence between the clades and the differences in architecture outside of the domain in some clades warns against such a conclusion.No member of this subfamily is characterised with respect to function, however the MDP-1 protein [ 1 ] is a characterised phosphatase. All of the characterised enzymes within subfamily III are phosphatases, and all of the active site residues characteristic of HAD-superfamily phosphatases [ 2 ] are present in subfamily IIIC.

1. MDP-1 is a new and distinct member of the haloacid dehalogenase family of aspartate-dependent phosphohydrolases. Biochemistry 40, 12704-11
2. Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. Application of an iterative approach to database search. J. Mol. Biol. 244, 125-32