InterPro domain: IPR007214
General Information
- Identifier IPR007214
- Description YbaK/aminoacyl-tRNA synthetase-associated domain
- Number of genes 229
- Gene duplication stats Loading...
- Associated GO terms GO:0002161
Abstract
Bacterial prolyl-tRNA synthetases and some smaller paralogues, YbaK and ProX, can hydrolyse misacylated Cys-tRNA(Pro) or Ala-tRNA(Pro) [ 1 ]. The small bacterial protein Ybak preferentially hydrolyses Cys-tRNA(Pro) and Cys-tRNA(Cys) [ 2 , 2 ]. ProX functions in trans to edit the amino acid moiety from incorrectly charged Ala-tRNA(Pro) [ 3 ]. Prolyl-tRNA synthetases main function is to catalyse the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) [ 4 ].
This entry represents a domain characteristic of Ybak and ProX, that can also be found with other domains in prolyl-tRNA synthetases.
1. The bacterial YbaK protein is a Cys-tRNAPro and Cys-tRNA Cys deacylase. J. Biol. Chem. 280, 25887-91
2. Aminoacyl-tRNA Substrate and Enzyme Backbone Atoms Contribute to Translational Quality Control by YbaK. J Phys Chem B 100, 15422-7
3. Trans-editing of mischarged tRNAs. Proc. Natl. Acad. Sci. U.S.A. 287, 479-82
4. One polypeptide with two aminoacyl-tRNA synthetase activities. Science