InterPro domain: IPR007174

Las1 is an endoribonuclease responsible for cleavage at the C2 site during pre-rRNA processing [ 1 ]. It interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis [ 2 ]. Las1-mediated C2 cleavage depends on the functional integrity of the associated Grc3 phosphotransferase, while phosphorylation of the 26S pre-rRNA by Grc3 relies on a functionally competent Las1 nuclease [ 3 , 4 ].

1. Characterization of the molecular crosstalk within the essential Grc3/Las1 pre-rRNA processing complex. RNA 24, 721-738
2. Las1 interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis in Saccharomyces cerevisiae. Nucleic Acids Res. 41, 1135-50
3. Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. Proc. Natl. Acad. Sci. U.S.A. 114, E5530-E5538
4. Coordinated Ribosomal ITS2 RNA Processing by the Las1 Complex Integrating Endonuclease, Polynucleotide Kinase, and Exonuclease Activities. Mol. Cell 60, 808-815