InterPro domain: IPR006065

O-Glycosyl hydrolases ( 3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ 1 , 2 ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Family 41 encompasses cytokinin-beta-glucosidases. These enzymes hydrolysecytokinin glucosides, liberating free cytokinins [ 3 ] and contribute to root-inducing activity [ 4 ].

1. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92, 7090-4
2. Structures and mechanisms of glycosyl hydrolases. Structure 3, 853-9
3. The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates. EMBO J. 10, 2889-95
4. Nucleotide sequence of the rol region of the mikimopine-type root-inducing plasmid pRi1724. Biosci. Biotechnol. Biochem. 58, 548-51