InterPro domain: IPR005549

This entry represents the N-terminal domain of kinetochore protein Nuf2, which is part of the Ndc80 complex. This domain fold as a CH domain consisting of a four-helix bundle containing the parallel helices alphaA, alphaC, alphaE, and alphaG, similar to that in Ndc80. These CH domains from Nuf2 and Ndc80 forms the globular rod at one end the complex [ 1 , 2 ]. The ability of the Ndc80 complex to bind microtubules resides on the tightly packed CH domains from Nuf2 and Ndc80 [ 3 ]. This complex binds to the spindle and is required for chromosome segregation and spindle checkpoint activity [ 3 , 4 , 5 , 6 , 7 , 8 , 9 ].

1. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J. Cell Biol. 152, 349-60
2. Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex. Cell 133, 427-39
3. hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J. Cell Biol. 159, 549-55
4. Thiamine deficiency in children with severe gastro-enteritis. S. Afr. Med. J. 46, 2083-4
5. The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo. Curr. Biol. 14, 611-7
6. Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1. Chromosoma 113, 1-15
7. Timing and checkpoints in the regulation of mitotic progression. Dev. Cell 7, 45-60
8. Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites. Mol. Biol. Cell 16, 519-31
9. Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J. Biol. Chem. 282, 21415-24