InterPro domain: IPR004971
General Information
- Identifier IPR004971
- Description mRNA (guanine-N(7))-methyltransferase domain
- Number of genes 324
- Gene duplication stats Loading...
Abstract
This entry represents mRNA (guanine-N(7))-methyltransferase, which can either be found as a single domain protein, or as a domain within the mRNA-capping enzyme catalytic subunit.
mRNA (guanine-N(7))-methyltransferase methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. It binds RNA containing 5'-terminal GpppC [ 1 , 2 , 3 , 4 , 5 , 6 ]. Viral mRNA capping enzymes, meanwhile, are multidomain proteins that catalyse the first two reactions in the mRNA cap formation pathway. They are heterodimers consisting of a large (catalytic) and small subunit.
1. Cloning and characterization of mRNA capping enzyme and mRNA (Guanine-7-)-methyltransferase cDNAs from Xenopus laevis. Biochem. Biophys. Res. Commun. 268, 617-24
2. Yeast mRNA cap methyltransferase is a 50-kilodalton protein encoded by an essential gene. Mol. Cell. Biol. 15, 4167-74
3. Cloning and characterization of three human cDNAs encoding mRNA (guanine-7-)-methyltransferase, an mRNA cap methylase. Biochem. Biophys. Res. Commun. 251, 27-34
4. Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes. J. Biol. Chem. 273, 21443-6
5. Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes. J. Biol. Chem. 274, 16553-62
6. RAM/Fam103a1 is required for mRNA cap methylation. Mol. Cell 44, 585-96