InterPro domain: IPR004383

General Information

  • Identifier IPR004383
  • Description Ribosomal RNA large subunit methyltransferase RlmN/Cfr
  • Number of genes 307
  • Gene duplication stats Loading...
  • Associated GO terms GO:0008173   GO:0006364  

Abstract

This entry represents the RlmN family, that includes dual-specificity RNA methyltransferase RlmN and ribosomal RNA large subunit methyltransferase Cfr.

Dual-specificity RNA methyltransferase RlmN specifically methylates position 2 of adenine 2503 in 23S rRNA [ 1 ]. This nucleotide is located in a functionally important region of the ribosome, at the entrance to the nascent peptide exit tunnel, and may thus be involved in the interaction with the nascent peptide. It also methylates position 2 of adenine 37 in tRNAs. Unmodified tRNA is not a suitable substrate for RlmN, which suggests that RlmN works in a late step during tRNA maturation [ 2 , 2 , 3 ].

Ribosomal RNA large subunit methyltransferase Cfr specifically methylates position 8 of adenine 2503 in 23S rRNA. It can also methylate position 2 of A2503 after the primary methylation is complete, to form 2,8-dimethyladenosine. Cfr confers antibiotic resistance in bacteria. The antibiotic resistance is provided by methylation at the 8 position and is independent of methylation at the 2 position of A2503 [ 4 , 5 ].


1. The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA. RNA 14, 98-106
2. A radically different mechanism for S-adenosylmethionine-dependent methyltransferases. Science 332, 604-7
3. The Escherichia coli RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy. RNA 18, 1783-95
4. Identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri. Antimicrob. Agents Chemother. 44, 2530-3
5. Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria. RNA 15, 327-36

Species distribution

Gene table

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