InterPro domain: IPR003732

This family consists of D-aminoacyl-tRNA deacylase DTD, also known as D-Tyr-tRNA(Tyr) deacylase. It is an enzyme that cleaves misacetylated D-aminoacyl-tRNA molecules into free tRNAs and D-amino acids [ 1 , 2 ]. Cell growth inhibition by several D-amino acids can be explained by an in vivo production of D-aminoacyl-tRNA molecules.

The enzyme is found in bacteria and in eukaryotes but not in archea. It has a beta barrel-like fold structure and forms homodimers in which two surface cavities serve as the active site for tRNA binding [ 3 , 4 , 5 ].

1. Structure of crystalline D-Tyr-tRNA(Tyr) deacylase. A representative of a new class of tRNA-dependent hydrolases. J. Biol. Chem. 276, 47285-90
2. The dtd gene from Bacillus amyloliquefaciens encodes a putative d-tyrosyl-tRNA(Tyr) deacylase and is a selectable marker for Bacillus subtilis. Microbiol. Res. 171, 90-6
3. Metabolism of D-aminoacyl-tRNAs in Escherichia coli and Saccharomyces cerevisiae cells. J. Biol. Chem. 275, 32535-42
4. D-tyrosyl-tRNA(Tyr) metabolism in Saccharomyces cerevisiae. J. Biol. Chem. 275, 11626-30
5. Functional characterization of the D-Tyr-tRNATyr deacylase from Escherichia coli. J. Biol. Chem. 274, 19109-14