InterPro domain: IPR002219
General Information
- Identifier IPR002219
- Description Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
- Number of genes 837
- Gene duplication stats Loading...
- Associated GO terms GO:0035556
Abstract
Diacylglycerol (DAG) is an important second messenger. Phorbol esters (PE) are analogues of DAG and potent tumour promoters that cause a variety of physiological changes when administered to both cells and tissues. DAG activates a family of serine/threonine protein kinases, collectively known as protein kinase C (PKC) [ 1 ]. Phorbol esters can directly stimulate PKC. The N-terminal region of PKC, known as C1, has been shown [ 2 ] to bind PE and DAG in a phospholipid and zinc-dependent fashion. The C1 region contains one or two copies (depending on the isozyme of PKC) of a cysteine-rich domain, which is about 50 amino-acid residues long, and which is essential for DAG/PE-binding. The DAG/PE-binding domain binds two zinc ions; the ligands of these metal ions are probably the six cysteines and two histidines that are conserved in this domain.
1. The protein kinase C family. Eur. J. Biochem. 208, 547-57
2. Phorbol ester binding to protein kinase C requires a cysteine-rich zinc-finger-like sequence. Proc. Natl. Acad. Sci. U.S.A. 86, 4868-71