InterPro domain: IPR000836

This entry refers to the phosphoribosyl transferase (PRT) type I domain. PRTases catalyze the displacement of the alpha-1'-pyrophosphate of 5-phosphoribosyl-alpha1-pyrpphosphate (PRPP) by a nitrogen-containing nucleophile. The reaction products are an alpha-1 substituted ribose-5'-phosphate and a free pyrophosphate (PP). PRPP, an activated form of ribose-5-phosphate, is a key metabolite connecting nucleotide synthesis and salvage pathways. The type I PRTases are identified by a conserved PRPP binding motif which features two adjacent acidic residues surrounded by one or more hydrophobic residue. This domain is found in a range of diverse phosphoribosyl transferase enzymes and regulatory proteins of the nucleotide synthesis and salvage pathways, including adenine phosphoribosyltransferase 2.4.2.7 , hypoxanthine-guanine-xanthine phosphoribosyltransferase, hypoxanthine phosphoribosyltransferase 2.4.2.8 , ribose-phosphate pyrophosphokinase 2.7.6.1 , amidophosphoribosyltransferase 2.4.2.14 , orotate phosphoribosyltransferase 2.4.2.10 , uracil phosphoribosyltransferase 2.4.2.9 , and xanthine-guanine phosphoribosyltransferase 2.4.2.22 . In Arabidopsis, at the very N terminus of this domain is the P-Loop NTPase domain [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ].

1. Functional characterization of a gene encoding a dual domain for uridine kinase and uracil phosphoribosyltransferase in Arabidopsis thaliana. Plant Mol. Biol. 63, 465-77
2. The PRT protein family. Curr. Opin. Struct. Biol. 11, 733-9
3. A second pathway to degrade pyrimidine nucleic acid precursors in eukaryotes. J. Mol. Biol. 380, 656-66
4. Crystal structure of a predicted phosphoribosyltransferase (TT1426) from Thermus thermophilus HB8 at 2.01 A resolution. Protein Sci. 14, 823-7
5. Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism. J. Biomol. Struct. Dyn. 25, 589-97
6. Silencing of mouse Aprt is a gradual process in differentiated cells. Mol. Cell. Biol. 23, 4461-70
7. HPRT-APRT-deficient mice are not a model for lesch-nyhan syndrome. Hum. Mol. Genet. 5, 1607-10
8. Molecular, kinetic and thermodynamic characterization of Mycobacterium tuberculosis orotate phosphoribosyltransferase. Mol Biosyst 8, 572-86
9. Mammalian pyrimidine biosynthesis: fresh insights into an ancient pathway. J. Biol. Chem. 279, 33035-8
10. Structural features of the phosphoribosyltransferases and their relationship to the human deficiency disorders of purine and pyrimidine metabolism. CRC Crit. Rev. Biochem. 11, 1-34
11. Pyrimidine nucleotide biosynthesis in animals: genes, enzymes, and regulation of UMP biosynthesis. Annu. Rev. Biochem. 49, 253-79
12. Pyrimidine salvage pathway in Mycobacterium tuberculosis. Curr. Med. Chem. 18, 1286-98
13. Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors. Microbiol. Mol. Biol. Rev. 72, 266-300, table of contents