InterPro domain: IPR000100
General Information
- Identifier IPR000100
- Description Ribonuclease P
- Number of genes 2
- Gene duplication stats Loading...
- Associated GO terms GO:0004526 GO:0008033 GO:0000049
Abstract
Ribonuclease P ( 3.1.26.5 ) (RNase P) [ 1 , 2 , 3 ] is a site specific endonuclease that generates mature tRNAs by catalysing the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. In bacteria RNase P is known to be composed of two components: a large RNA (about 400 base pairs) encoded by rnpB, and a small protein (119 to 133 amino acids) encoded by rnpA. The RNA moiety of RNase P carries the catalytic activity; the protein component plays an auxiliary, but essential, role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.The sequence of rnpA is not highly conserved, however there is, in the centralpart of the protein, a conserved basic region.
1. Ribonuclease P: function and variation. J. Biol. Chem. 265, 3587-90
2. Ribonuclease P. Postscript. J. Biol. Chem. 265, 20053-6
3. Ribonuclease P RNA and protein subunits from bacteria. Nucleic Acids Res. 20, 1451-6