Gene: Sopen07g029520

General Information

Description Cytochrome P450 724B1 (EC 1.14.-.-) (OsDWARF11) (Dwarf protein 11) - Oryza sativa (Rice), Encodes a 22, #945 | hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway. The protein is a member of CYP90B gene family. CLM is an epi-allele with small, compressed rosette, reduced internode length, and reduced fertility, appears in selfed ddm mutant plants possibly due to loss of cytosine methylation. Transcripts accumulate in actively growing tissues, and GUS expression is negatively regulated by brassinosteroids. Localized in the endoplasmic reticulum. The in vitro expressed protein can perform the C-22 hydroxylation of a variety of C27-, C28- and C29-sterols. Cholesterol was the best substrate, followed by campesterol. Sitosterol was a poor substrate. | DWARF 4 (DWF4) | CONTAINS InterPro DOMAIN/s: Cytochrome P450 , Cytochrome P450, conserved site , Cytochrome P450, E-class, group I | BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein

GO term	Evidence(s)	Provider(s)	Description	Source(s)
GO:0007275	ISO	PLAZA Integrative Orthology	multicellular organism development	Os04g0469800
GO:0016132	ISO	PLAZA Integrative Orthology	brassinosteroid biosynthetic process	AT5G14400
GO:0009647	ISO	PLAZA Integrative Orthology	skotomorphogenesis	Os04g0469800

GO term	Evidence(s)	Provider(s)	Description	Source(s)
GO:0004497	IEA	InterPro	monooxygenase activity
GO:0005506	IEA	InterPro	iron ion binding
GO:0016705	IEA	InterPro	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037	IEA	InterPro	heme binding
GO:0010012	ISO	PLAZA Integrative Orthology	steroid 22-alpha hydroxylase activity	AT5G14400

Experimental Evidence

Computational Reviewed Evidence

Electronic Evidence

Mapman id	Description
50.1.13	Enzyme classification.EC_1 oxidoreductases.EC_1.14 oxidoreductase acting on paired donor with incorporation or reduction of molecular oxygen