Gene: Sopen02g030050

General Information

Structural Information

  • Species Solanum pennellii
  • Gene Identifier Sopen02g030050
  • Transcript Identifier Sopen02g030050.1
  • Gene Type Coding gene
  • Location Spenn-ch02 : 52246550-52250659 : negative

Gene Family Information

  • ID HOM05D000093
  • #Genes/#Species 3423/99
  • Phylogenetic origin

Gene Duplication Information

Labels

Identifiers

  • tid Sopen02g030050.1

Descriptions

  • Description Encodes a 22, #945 | hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway. The protein is a member of CYP90B gene family. CLM is an epi-allele with small, compressed rosette, reduced internode length, and reduced fertility, appears in selfed ddm mutant plants possibly due to loss of cytosine methylation. Transcripts accumulate in actively growing tissues, and GUS expression is negatively regulated by brassinosteroids. Localized in the endoplasmic reticulum. The in vitro expressed protein can perform the C-22 hydroxylation of a variety of C27-, C28- and C29-sterols. Cholesterol was the best substrate, followed by campesterol. Sitosterol was a poor substrate. | DWARF 4 (DWF4) | CONTAINS InterPro DOMAIN/s: Cytochrome P450 , Cytochrome P450, conserved site , Cytochrome P450, E-class, group I | BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein
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Functional Annotation

Biological Process

GO termEvidence(s)Provider(s)DescriptionSource(s)
GO:0009826
ISO
PLAZA Integrative Orthologyunidimensional cell growth AT3G50660
GO:0007275
ISO
PLAZA Integrative Orthologymulticellular organism development Os03g0227700
GO:0048366
ISO
PLAZA Integrative Orthologyleaf development AT3G50660
GO:0010358
ISO
PLAZA Integrative Orthologyleaf shaping AT3G50660
GO:0009753
ISO
PLAZA Integrative Orthologyresponse to jasmonic acid AT3G50660
GO:0016132
ISO
PLAZA Integrative Orthologybrassinosteroid biosynthetic process AT3G50660
GO:0009741
ISO
PLAZA Integrative Orthologyresponse to brassinosteroid AT3G50660
GO:0009867
ISO
PLAZA Integrative Orthologyjasmonic acid mediated signaling pathway AT3G50660

Molecular Function

GO termEvidence(s)Provider(s)DescriptionSource(s)
GO:0004497
IEA
InterPromonooxygenase activity
GO:0005506
IEA
InterProiron ion binding
GO:0016705
IEA
InterProoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037
IEA
InterProheme binding
GO:0080132
ISO
PLAZA Integrative Orthologyfatty acid alpha-hydroxylase activity Os03g0227700
GO:0010012
ISO
PLAZA Integrative Orthologysteroid 22-alpha hydroxylase activity AT3G50660

Cellular Component

GO termEvidence(s)Provider(s)DescriptionSource(s)
GO:0005783
ISO
PLAZA Integrative Orthologyendoplasmic reticulum AT3G50660

Color Legend

Experimental Evidence
Computational Reviewed Evidence
Electronic Evidence
GO Sources: Primary Orthology Homology
Show redundant parents:
InterPro Description
IPR001128 Cytochrome P450
IPR036396 Cytochrome P450 superfamily
IPR002403 Cytochrome P450, E-class, group IV
Mapman id Description
11.3.1.1 Phytohormone action.brassinosteroid.biosynthesis.steroid 22-alpha-hydroxylase (DWF4)