PP2A-3 interacts with ACR4 and regulates formative cell division in the Arabidopsis root

In plants, the generation of new cell types and tissues depends on coordinated and oriented formative cell divisions. The plasma membrane-localized receptor kinase ARABIDOPSIS CRINKLY 4 (ACR4) is part of a mechanism controlling formative cell divisions in the Arabidopsis root. Despite its important role in plant development, very little is known about the molecular mechanism ACR4 is affiliated to and its network of interactions. Here, we used various complementary proteomic approaches to identify ACR4-interacting protein candidates that are likely novel regulators of formative cell divisions and that could pave the way to unraveling the molecular basis behind ACR4-mediated signaling. We identified PROTEIN PHOSPHATASE 2A-3 (PP2A-3), a catalytic subunit of PP2A holoenzymes, as a novel regulator of formative cell divisions and as the first described substrate of ACR4. Our in vitro data argue for the existence of a tight post-translational regulation in the associated biochemical network through reciprocal regulation between ACR4 and PP2A-3 at the phosphorylation level.

Yue, K., Sandal, P., Williams, C., Murphy, E., Stest, E., Nikonovora, N., Ramakrishna, P., Czyzewicz, N., Montero Morales, L., Kumpf, R., Lin, Z., Van de Cotte, B., Iqbal, M., Van Bel, M., Van De Slijke, E., Meyer, M.R., Gadeyne, A., Zipfel, C., De Jaeger, G., Van Montagu, M., Van Damme, D., Gevaert, O., Rao, A.G., Beeckman, T., De Smet, I. (2015) PP2A-3 interacts with ACR4 and regulates formative cell division in the Arabidopsis root. PNAS 113(5):1447-52.

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