PLAZA 4.0 Monocots gene overview : AT3G44310

Gene: AT3G44310 (Arabidopsis thaliana)

Curated Summary: Mutants are resistant to indole-3-acetonitrile (IAN). NIT1 catalyzes the terminal activation step in indole-acetic acid biosynthesis. Predominantly expressed isoform of nitrilase isoenzyme family. Aggregation of NIT1 in cells directly abutting wound sites is one of the earliest events associated with wound and herbicide-induced cell death. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide. It is also involved in the conversion of IAN to IAM (indole-3-acetamide) and other non-auxin-related metabolic processes.

Type	Value
Alias	ATNIT1,A. THALIANA NITRILASE 1,NITI
symbol	NIT1
tid	AT3G44310.1
UniProt	P32961

GO term	Evidence(s)	Provider	Description	Source
GO:0006807	IEA	UniProt	nitrogen compound metabolic process	1
GO:0009684	TAS	UniProt	indoleacetic acid biosynthetic process	1
GO:0009617	IEA	PLAZA Integrative Orthology	response to bacterium	AT3G44300
GO:0051410	IEA	PLAZA Tree-based Orthology	detoxification of nitrogen compound	AT5G22300
GO:0009970	IEA	PLAZA Integrative Orthology	cellular response to sulfate starvation	AT3G44320
GO:0019762	IEA	PLAZA Integrative Orthology	glucosinolate catabolic process	AT3G44320
GO:0046686	IEA	PLAZA Integrative Orthology	response to cadmium ion	AT3G44300
GO:0019499	IEA	PLAZA Tree-based Orthology	cyanide metabolic process	AT5G22300

GO term	Evidence(s)	Provider	Description	Source
GO:0016810	IEA	UniProt	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	1
GO:0000257	IDA	UniProt	nitrilase activity	1
GO:0080109	IDA	UniProt	indole-3-acetonitrile nitrile hydratase activity	1
GO:0080061	IDA	UniProt	indole-3-acetonitrile nitrilase activity	1
GO:0047558	IEA	PLAZA Tree-based Orthology	3-cyanoalanine hydratase activity	AT5G22300
GO:0018822	IEA	PLAZA Tree-based Orthology	nitrile hydratase activity	AT5G22300
GO:0047427	IEA	PLAZA Tree-based Orthology	cyanoalanine nitrilase activity	AT5G22300

GO term	Evidence(s)	Provider	Description	Source
GO:0005886	IDA, ISM,	UniProt	plasma membrane	1 2
GO:0009507	IDA	UniProt	chloroplast	1
GO:0048046	IDA	UniProt	apoplast	1
GO:0005829	IDA	UniProt	cytosol	1
GO:0009506	IDA	UniProt	plasmodesma	1

Experimental Evidence

Electronic Evidence

Computational Reviewed Evidence

InterPro	Description
IPR003010	Carbon-nitrogen hydrolase
IPR000132	Nitrilase/cyanide hydratase, conserved site

Mapman id	Description
9.5.2.5	.Secondary metabolism.glucosinolates.glucosinolate degradation.nitrilase

No SignalP domains detected for this gene.