This domain forms the central core of Tn5-type transposase proteins. Prokaryotic Tn5 transposase makes two types of DNA contacts (cis-contacts for DNA recognition and trans-contacts for catalysis) as well as protein-protein contacts [1]. Tn5 transposase employs two cations at its active site during catalysis, which cause conformational changes in the bound DNA and which are required for transposition [2]. Tn5 transposase has a RnaseH-type alpha/beta/alpha fold. The central core domain has an alpha/beta complex structure.

1. Structure/function insights into Tn5 transposition. Curr. Opin. Struct. Biol. 14, 50-7
2. Two-metal active site binding of a Tn5 transposase synaptic complex. Nat. Struct. Biol. 9, 278-81

This domain forms the central core of Tn5-type transposase proteins. Prokaryotic Tn5 transposase
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