Gene: AT3G30180 (Arabidopsis thaliana)

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Gene Identifier
AT3G30180
Transcript Identifier
AT3G30180.1
Gene Type
Coding gene
Location
3 : 11810867-11813509 : positive

Family

Gene family
HOM03M000086
(513 genes in 16 species)
specific family
Duplication type
Tandem duplicate

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Descriptions

Description
brassinosteroid-6-oxidase 2
Curated Summary
Encodes a cytochrome p450 enzyme that catalyzes the last reaction in the production of brassinolide. It is capable of converting 6-deoxocastasterone into castasterone, a C-6 oxidation, as well as the further conversion of castasterone into brassinolide by a Baeyer-Villinger oxidation reaction at C-6, resulting in the formation of an unusual seven-membered lactone ring. The enzyme possesses high affinity for both C28- and C27-Brassinosteroids. The expression of the gene using a CYP85A2 promoter:LUC fusion construct was shown to be under circadian and light control.
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Identifiers

Identifier Name
aliasCYP85A2
aliasBR6OX2
aliasbrassinosteroid-6-oxidase 2
uniprotQ940V4

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Biological Process

GO termEvidence(s)ProviderDescriptionSource
GO:0016132IDAUniProtbrassinosteroid biosynthetic process1 2 3 4
GO:0055114IEAInterProoxidation-reduction process
GO:0010268ISOPLAZA Integrative Orthologybrassinosteroid homeostasisOS03G40540
GO:0001578ISOPLAZA Integrative Orthologymicrotubule bundle formationOS03G40540
GO:0009647ISOPLAZA Integrative OrthologyskotomorphogenesisOS03G40540
GO:0044699IEAPLAZA Homologysingle-organism processHOM03M000086
GO:0044710IEAPLAZA Homologysingle-organism metabolic processHOM03M000086

Molecular Function

GO termEvidence(s)ProviderDescriptionSource
GO:0019825ISSGene Ontologyoxygen binding1
GO:0004497IDAUniProtmonooxygenase activity1 2 3 4
GO:0020037IEAUniProtheme binding1 2 3
GO:0005506IEAUniProtiron ion binding1 2 3
GO:0016705IEAUniProtoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen1 2 3
GO:0043169IEAPLAZA Homologycation bindingHOM03M000086
GO:0043167IEAPLAZA Homologyion bindingHOM03M000086
GO:0046914IEAPLAZA Homologytransition metal ion bindingHOM03M000086
GO:0046906IEAPLAZA Homologytetrapyrrole bindingHOM03M000086
GO:0046872IEAPLAZA Homologymetal ion bindingHOM03M000086
GO:1901363IEAPLAZA Homologyheterocyclic compound bindingHOM03M000086
GO:0016491IEAPLAZA Homologyoxidoreductase activityHOM03M000086
GO:0097159IEAPLAZA Homologyorganic cyclic compound bindingHOM03M000086

Cellular Component

GO termEvidence(s)ProviderDescriptionSource
GO:0005576ISMGene Ontologyextracellular region1
GO:0016021IEAUniProtintegral to membrane1 2 3

Color Legend

Experimental Evidence
Electronic Evidence
Computational Reviewed Evidence
GO Sources:   Primary     Orthology     Homology  
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InterPro Description
IPR001128Cytochrome P450
IPR002401Cytochrome P450, E-class, group I
IPR017972Cytochrome P450, conserved site

Mapman id Description
17.3.1.1.99hormone metabolism.brassinosteroid.synthesis-degradation.BRs.other
SignalP Description
SignalP-TMSignal Peptide detected using TM network