1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (also known as MTCBP-1) (1.13.11.54) is an eukaryotic enzyme that catalyses the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). It shows significant homology to the bacterial aci-reductone dioxygenase (ARD), which is an enzyme in the methionine salvage pathway (MTA cycle) [1, 2]. MTCBP-1 is necessary for hepatitis C virus replication in an otherwise non-permissive cell line [3]. MTCBP-1 interacts and inhibits the activity of membrane-type 1 matrix metalloproteinase (MT1-MMP/MMP-14) in promoting tumor cell migration and invasion [4].

MTCBP-1 contains the cupin sequence motif and belongs to the Cupin superfamily composed of proteins with diverse functions [4].

1. Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway. Genes Cells 10, 565-74
2. Thiol dioxygenases: unique families of cupin proteins. Amino Acids 41, 91-102
3. Identification of a hepatic factor capable of supporting hepatitis C virus replication in a nonpermissive cell line. J. Virol. 75, 11017-24

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (also known as MTCBP-1) (Show more...

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