Gene: AT1G03310 (Arabidopsis thaliana)

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Gene Identifier
AT1G03310
Transcript Identifier
AT1G03310.2
Gene Type
Coding gene
Location
1 : 813975-816623 : positive

Family

Gene family
HOM03D000937
(156 genes in 31 species)
specific family
Subfamily
ORTHO03D014579
(14 genes in 14 species)
specific family

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Descriptions

Description
debranching enzyme 1
Curated Summary
Encodes a protein with strong similarity to isoamylase (EC:3.2.1.68) however lacks critical residues known to be important for activity. Appears to co localize with ISA1 in the chloroplast isoamylase complex. Mutations in this gene cause the loss of detectable isoamylase activity and the disruption of normal starch structure. It has been postulated that AtISA2 interacts with AtISA1 to form the Iso1 complex.
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Identifiers

Identifier Name
aliasISA2
aliasARABIDOPSIS THALIANA ISOAMYLASE 2
aliasDBE1
aliasBE2
aliasATISA2
aliasAtBE2
aliasdebranching enzyme 1
aliasBRANCHING ENZYME 2
uniprotQ8L735

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Biological Process

GO termEvidence(s)ProviderDescriptionSource
GO:0005975ISS, IEAGene Ontologycarbohydrate metabolic process1
GO:0010021IMPUniProtamylopectin biosynthetic process1 2 3 4 5 6 7 8
GO:0000023RCAGene Ontologymaltose metabolic process1
GO:0019252IEA, RCAUniProtstarch biosynthetic process1 2 3 4 5 6 7 8
GO:0019761RCAGene Ontologyglucosinolate biosynthetic process1
GO:0043085RCAGene Ontologypositive regulation of catalytic activity1

Molecular Function

GO termEvidence(s)ProviderDescriptionSource
GO:0004556ISSGene Ontologyalpha-amylase activity1
GO:0019156IDA, IMP, UniProtisoamylase activity1 2 3 4 5 6 7 8 9
GO:0043169IEAUniProtcation binding1 2 3 4 5 6 7
GO:0003824IEAInterProcatalytic activity
GO:0004553IEAInterProhydrolase activity, hydrolyzing O-glycosyl compounds

Cellular Component

GO termEvidence(s)ProviderDescriptionSource
GO:0009507IDAUniProtchloroplast1 2 3 4 5 6 7

Color Legend

Experimental Evidence
Electronic Evidence
Computational Reviewed Evidence
GO Sources:   Primary     Orthology     Homology  
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InterPro Description
IPR006589Glycosyl hydrolase, family 13, subfamily, catalytic domain
IPR013780Glycosyl hydrolase, family 13, all-beta
IPR006047Glycosyl hydrolase, family 13, catalytic domain
IPR014756Immunoglobulin E-set
IPR004193Glycoside hydrolase, family 13, N-terminal
IPR013783Immunoglobulin-like fold
IPR017853Glycoside hydrolase, superfamily
IPR015902Glycoside hydrolase, family 13
IPR013781Glycoside hydrolase, catalytic domain

Mapman id Description
2.1.2.4major CHO metabolism.synthesis.starch.debranching
No SignalP domains detected for this gene.