Gene: AT3G50660 (Arabidopsis thaliana)

Overview

Gene Identifier
AT3G50660
Transcript Identifier
AT3G50660.1
Gene Type
Coding gene
Location
3 : 18814262-18817168 : negative

Family

Gene family
HOM03D000054
(839 genes in 16 species)
specific family
Subfamily
ORTHO03D017117
(5 genes in 5 species)
specific family

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Descriptions

Description
Cytochrome P450 superfamily protein
Curated Summary
Encodes a 22α hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway. The protein is a member of CYP90B gene family. CLM is an epi-allele with small, compressed rosette, reduced internode length, and reduced fertility, appears in selfed ddm mutant plants possibly due to loss of cytosine methylation. Transcripts accumulate in actively growing tissues, and GUS expression is negatively regulated by brassinosteroids. Localized in the endoplasmic reticulum. The in vitro expressed protein can perform the C-22 hydroxylation of a variety of C27-, C28- and C29-sterols. Cholesterol was the best substrate, followed by campesterol. Sitosterol was a poor substrate.
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Identifiers

Identifier Name
aliasSHADE AVOIDANCE 1
aliasSUPPRESSOR OF NPH4 2
aliasPSC1
aliasDWARF 4
aliasCLOMAZONE-RESISTANT
aliasCLM
aliasDWF4
aliasSNP2
aliasPARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1
aliasCYTOCHROME P450 90B1
aliasAtDWF4
aliasSAV1
aliasCYP90B1
uniprotO64989

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Biological Process

GO termEvidence(s)ProviderDescriptionSource
GO:0009741IMP, IEPUniProtresponse to brassinosteroid1 2 3 4 5 6
GO:0010358IMPUniProtleaf shaping1 2 3 4 5
GO:0048366IMPGene Ontologyleaf development1
GO:0009753IMPGene Ontologyresponse to jasmonic acid1
GO:0009867IGIUniProtjasmonic acid mediated signaling pathway1 2 3 4 5
GO:0016132IMP, RCA, UniProtbrassinosteroid biosynthetic process1 2 3 4 5 6
GO:0016126RCAGene Ontologysterol biosynthetic process1
GO:0009826IMPUniProtunidimensional cell growth1 2 3 4 5
GO:0055114IEAInterProoxidation-reduction process
GO:0044699IEAPLAZA Homologysingle-organism processHOM03D000064
GO:0044710IEAPLAZA Homologysingle-organism metabolic processHOM03D000064
GO:0008152IEAPLAZA Homologymetabolic processHOM03D000064

Molecular Function

GO termEvidence(s)ProviderDescriptionSource
GO:0010012IDAUniProtsteroid 22-alpha hydroxylase activity1 2 3 4 5
GO:0005506IEAUniProtiron ion binding1 2 3 4
GO:0016705IEAUniProtoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen1 2 3 4
GO:0020037IEAUniProtheme binding1 2 3 4
GO:0043169IEAPLAZA Homologycation bindingHOM03D000064
GO:0043167IEAPLAZA Homologyion bindingHOM03D000064
GO:0046914IEAPLAZA Homologytransition metal ion bindingHOM03D000064
GO:0003824IEAPLAZA Homologycatalytic activityHOM03D000064
GO:0046906IEAPLAZA Homologytetrapyrrole bindingHOM03D000064
GO:0046872IEAPLAZA Homologymetal ion bindingHOM03D000064
GO:1901363IEAPLAZA Homologyheterocyclic compound bindingHOM03D000064
GO:0005488IEAPLAZA HomologybindingHOM03D000064
GO:0016491IEAPLAZA Homologyoxidoreductase activityHOM03D000064
GO:0097159IEAPLAZA Homologyorganic cyclic compound bindingHOM03D000064

Cellular Component

GO termEvidence(s)ProviderDescriptionSource
GO:0005783IDAUniProtendoplasmic reticulum1 2 3 4 5
GO:0016021IEAUniProtintegral to membrane1 2 3 4

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Computational Reviewed Evidence
Electronic Evidence
Experimental Evidence
GO Sources:   Primary     Orthology     Homology  
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InterPro Description
IPR002401Cytochrome P450, E-class, group I
IPR001128Cytochrome P450
IPR017972Cytochrome P450, conserved site
Mapman id Description
17.3.1.1.2hormone metabolism.brassinosteroid.synthesis-degradation.BRs.DWF4
No SignalP domains detected for this gene.