InterPro domain: IPR023208

This group represents the NADPH--cytochrome P450 reductase (CPR) from eukaryotes. CPR is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5 [ 1 , 2 ].

Interestingly, animals harbour a single CPR gene, while higher plants have multiple CPRs with as many as three in Populus tricocarpa and Oryza sativa [ 3 ]. In comparison with other CPRs, plant CPRs often possess long N-terminal membrane-spanning domains [ 4 ].

1. Cloning and characterization of the NADPH cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger. DNA Cell Biol. 14, 719-29
2. Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger. Arch. Biochem. Biophys. 394, 245-54
3. Rice P450 reductases differentially affect P450-mediated metabolism in bacterial expression systems. Bioprocess Biosyst Eng 36, 325-31
4. Plant NADPH-cytochrome P450 oxidoreductases. Phytochemistry 71, 132-41