| AT3G30180 | brassinosteroid-6-oxidase 2 (BR6OX2); FUNCTIONS IN: monooxygenase activity, oxygen binding; INVOLVED IN: response to light stimulus, circadian rhythm, brassinosteroid biosynthetic process; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: brassinosteroid-6-oxidase 1 (TAIR:AT5G38970.1); Has 29372 Blast hits to 29308 proteins in 1622 species: Archae - 48; Bacteria - 4489; Metazoa - 10449; Fungi - 5456; Plants - 7735; Viruses - 3; Other Eukaryotes - 1192 (source: NCBI BLink). brassinosteroid-6-oxidase 2 Encodes a cytochrome p450 enzyme that catalyzes the last reaction in the production of brassinolide. It is capable of converting 6-deoxocastasterone into castasterone, a C-6 oxidation, as well as the further conversion of castasterone into brassinolide by a Baeyer-Villinger oxidation reaction at C-6, resulting in the formation of an unusual seven-membered lactone ring. The enzyme possesses high affinity for both C28- and C27-Brassinosteroids. The expression of the gene using a CYP85A2 promoter:LUC fusion construct was shown to be under circadian and light control. | 3 | + | View |
| AT5G38970 | brassinosteroid-6-oxidase 1 Encodes a polypeptide involved in the C-6 oxidation of brassinosteroids. Heterologous expression of the protein in yeast conferred the ability to catalyze multiple reactions in which the C-6 position of 6-deoxocastasterone, 6-deoxotyphasterol, 3-dehydro-6-deoxoteasterone and 6-deoxoteasterone are oxidized. brassinosteroid-6-oxidase 1 (BR6OX1); FUNCTIONS IN: monooxygenase activity, oxygen binding; INVOLVED IN: brassinosteroid homeostasis, brassinosteroid biosynthetic process; LOCATED IN: endomembrane system; EXPRESSED IN: fruit; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group IV (InterPro:IPR002403), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: brassinosteroid-6-oxidase 2 (TAIR:AT3G30180.1); Has 28933 Blast hits to 28862 proteins in 1616 species: Archae - 48; Bacteria - 4699; Metazoa - 10231; Fungi - 5134; Plants - 7576; Viruses - 3; Other Eukaryotes - 1242 (source: NCBI BLink). | 5 | - | View |
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