Joanna Boruc, Hilde Van den Daele, Jens Hollunder, Stephane Rombauts, Evelien Mylle, Pierre Hilson, Dirk Inzé, Lieven De Veylder, and Eugenia Russinova
As in other eukaryotes, cell division in plants is highly conserved and controlled by cyclin-dependent kinases that are predominantly regulated at the post-transcriptional level by their association with regulatory proteins, such as cyclins. Although over the last years the knowledge of the plant cell cycle has considerably increased, little is known on the assembly and regulation of the different CDK complexes. To map protein-protein interactions between core cell cycle proteins of Arabidopsis thaliana, a binary protein-protein interactome network was generated using two complementary high-throughput interaction assays, yeast two-hybrid and bimolecular fluorescence complementation. Pairwise interactions among 58 core cell cycle proteins were tested, resulting into 357 interactions of which 293 not reported before. Integration of the binary interaction results with cell cycle phase-dependent expression information and localization data allowed the construction of a dynamic interaction network. The obtained interaction map constitutes a framework for further in-depth analysis of the cell cycle machinery.